Etude épidémiologique des mycoplasmes des petits ruminants.

Abstract

An epidemiologic study on mycoplasmas of sheep, was conducted for the first time in Algeria M.agalactiae , M.capricolum, M.ovipneumoniae and M.arginini were found in animals with symptoms and lesions respiratory. A serological investigation by F.C. and E.L.I.S.A. revealed the presence of antibodies against mycoplasma. Furthermore, this strains were identified by biochemical test (arginin hydrolysis, tetrazolium reduction, film and spots and phosphatase) and serologic(inhibition of growth in antiserums presence) . The mycoplasma are the origine of great pathology at the ruminants. To better elucidate the mecanism of persistency of these bacterial in the animal organism, two experiments had been realised with P89 strains (M.agalactiae ) and differents doses. During the two experiments, specific serum antibodies were detected by western blot, determining a few immunodominant antigen recognised by the immunserums wich will survive for the further studies in the approach vaccination.